Journal: Scientific reports

Article Title: Der p 1-based immunotoxin as potential tool for the treatment of dust mite respiratory allergy.

doi: 10.1038/s41598-020-69166-w

Figure Lengend Snippet: Figure 1. Domain arrangement of proDerp1αS construct, along with SDS-PAGE, Western blot and mass spectrometry analysis of the purified immunotoxin. (A) Schematic representation of proDerp1αS cDNA, highlighting its structural and functional motifs, alongside its theoretical molecular mass. 3D-model structure of proDerp1αS is included as Figure S1. (B) Coomassie blue stained SDS-PAGE (CBS) and Western blot analysis using rabbit anti-α-sarcin (left) and anti-α-Der p 1 antisera (right). CBS protein molecular weight standards correspond to Bio-Rad Unstained SDS-PAGE low range Standards; while the prestained Bio-Rad Precision. Plus Dual Color Standards (Bio-Rad, Hercules, CA, USA) were used for Western blot. Images correspond to full-length gels and blots acquired and analyzed using the Gel Doc XR Imaging System and Quantity One 1-D analysis sofware (BioRad) or ChemiDoc-It (UVP) and VisionWorks LS, respectively.Full-length blots/gels are presented in Supplementary Figure S2. (C) Mass spectrometry analysis spectrum showing the corresponding peak to proDerp1αS construct. The difference between theoretical and empirical masses agrees with the four N-terminal extra amino acids of α-factor secretion signal that could remain after its processing by kex2 protease.

Article Snippet: CBS protein molecular weight standards correspond to Bio-Rad Unstained SDS-PAGE low range Standards; while the prestained Bio-Rad Precision.

Techniques: Construct, SDS Page, Western Blot, Mass Spectrometry, Purification, Functional Assay, Staining, Molecular Weight, Imaging

Journal: Biology

Article Title: Isolation and Characterization a Novel Catabolic Gene Cluster Involved in Chlorobenzene Degradation in Haloalkaliphilic Alcanivorax sp. HA03

doi: 10.3390/biology11050724

Figure Lengend Snippet: SDS-PAGE analysis of cell extracts of E. coli JM109 (lane 1), E. coli JM109 (pUC119) (lane 2), E. coli JM109 ( pCB ) pregrown in the presence of IPTG (lane 3) and E. coli JM109 ( pCB ) grown in the absence of IPTG (lane 4). In total, 10 µL of extracts were subjected to SDS-PAGE., M PageRulerTM Prestained protein ladder (Thermo Scientific).

Article Snippet: The denaturated protein subunits was evaluated for its molecular weight using the PageRulerTM Prestained protein ladder (Thermo Scientific, Waltham, MA, USA).

Techniques: SDS Page

Journal: Biology

Article Title: Isolation and Characterization a Novel Catabolic Gene Cluster Involved in Chlorobenzene Degradation in Haloalkaliphilic Alcanivorax sp. HA03

doi: 10.3390/biology11050724

Figure Lengend Snippet: SDS-PAGE analysis of cell extracts of E. coli JM109 with CC12Dos gene insert diluted 1:1 (lane 1) and 1:10 (lane 2); M PageRuler™ Prestained 10–170 kDa (Thermo Scientific).

Article Snippet: The denaturated protein subunits was evaluated for its molecular weight using the PageRulerTM Prestained protein ladder (Thermo Scientific, Waltham, MA, USA).

Techniques: SDS Page